The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice
RM Martin, JL Brady, AM Lew - Journal of immunological methods, 1998 - Elsevier
Isotyping and quantitation of murine IgG2a antibodies are widely performed with commercial
monoclonal and polyclonal antisera raised against BALB/c IgG2a myeloma proteins.
Recently it became evident that inbred mouse strains with the Igh1-b allele do not have the
gene for IgG2a and instead express the IgG2c isotype. We show that commercial anti-IgG2a
sera cross-react inadequately against IgG2c in immunoblot and ELISA and hence, are not
suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 …
monoclonal and polyclonal antisera raised against BALB/c IgG2a myeloma proteins.
Recently it became evident that inbred mouse strains with the Igh1-b allele do not have the
gene for IgG2a and instead express the IgG2c isotype. We show that commercial anti-IgG2a
sera cross-react inadequately against IgG2c in immunoblot and ELISA and hence, are not
suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 …
