[CITATION][C] Histochemical differentiation during neutrophil development and maturation

GA Ackerman - Annals of the New York Academy of Sciences, 1964 - Wiley Online Library
GA Ackerman
Annals of the New York Academy of Sciences, 1964Wiley Online Library
Lipids. Sudan black B (National Aniline) in both 70 per cent ethanol and in proplyene glycol
was used for the localization of lipid and phospholipid after formalin vapor fixation; the
plasma1 reaction for plasmalogen after formalin vapor fixation (GA Ackerman, 1963u; Mota
et al., 1952); the Baker acid hematin test was used for the demonstration of phospholipids as
detailed by ML Bloom and GB Wislocki (1950). Hydrolytic enzymes. Naphthol AS-MX
phosphate-Fast blue RR technique was employed for the demonstration of alkaline …
Lipids. Sudan black B (National Aniline) in both 70 per cent ethanol and in proplyene glycol was used for the localization of lipid and phospholipid after formalin vapor fixation; the plasma1 reaction for plasmalogen after formalin vapor fixation (GA Ackerman, 1963u; Mota et al., 1952); the Baker acid hematin test was used for the demonstration of phospholipids as detailed by ML Bloom and GB Wislocki (1950).
Hydrolytic enzymes. Naphthol AS-MX phosphate-Fast blue RR technique was employed for the demonstration of alkaline phosphatase activity using cold formalin-methanol fixation (GA Ackerman, 1962~). Adenosine triphosphatase (ATPase) activity or a closely related enzyme system was localized using the Wachstein-Meisel procedure (AG E. Pearse, 1960) after brief fixation in formal vapor, formal-calcium, or cold 95 per cent acetone. Nonspecific esterase
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