[PDF][PDF] Pdgfrβ+ mural preadipocytes contribute to adipocyte hyperplasia induced by high-fat-diet feeding and prolonged cold exposure in adult mice
Cell metabolism, 2016•cell.com
The expansion of white adipose tissue (WAT) in obesity involves de novo differentiation of
new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize
Zfp423 GFP reporter mice to characterize adipose mural (Pdgfrβ+) cells with varying levels
of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct
mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like
mural cells. Using our" MuralChaser" lineage tracking system, we uncover adipose …
new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize
Zfp423 GFP reporter mice to characterize adipose mural (Pdgfrβ+) cells with varying levels
of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct
mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like
mural cells. Using our" MuralChaser" lineage tracking system, we uncover adipose …
Summary
The expansion of white adipose tissue (WAT) in obesity involves de novo differentiation of new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize Zfp423GFP reporter mice to characterize adipose mural (Pdgfrβ+) cells with varying levels of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like mural cells. Using our "MuralChaser" lineage tracking system, we uncover adipose perivascular cells as developmental precursors of adipocytes formed in obesity, with adipogenesis and precursor abundance regulated in a depot-dependent manner. Interestingly, Pdgfrβ+ cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. These results provide genetic evidence for a mural cell origin of white adipocytes in obesity and suggest that beige adipogenesis may originate from multiple sources.
cell.com