[HTML][HTML] Tyrosine 192 in apolipoprotein AI is the major site of nitration and chlorination by myeloperoxidase, but only chlorination markedly impairs ABCA1-dependent …
High density lipoprotein (HDL) isolated from human atherosclerotic lesions and the blood of
patients with established coronary artery disease contains elevated levels of 3-nitrotyrosine
and 3-chlorotyrosine. Myeloperoxidase (MPO) is the only known source of 3-chlorotyrosine
in humans, indicating that MPO oxidizes HDL in vivo. In the current studies, we used tandem
mass spectrometry to identify the major sites of tyrosine oxidation when lipid-free
apolipoprotein AI (apoA-I), the major protein of HDL, was exposed to MPO or peroxynitrite …
patients with established coronary artery disease contains elevated levels of 3-nitrotyrosine
and 3-chlorotyrosine. Myeloperoxidase (MPO) is the only known source of 3-chlorotyrosine
in humans, indicating that MPO oxidizes HDL in vivo. In the current studies, we used tandem
mass spectrometry to identify the major sites of tyrosine oxidation when lipid-free
apolipoprotein AI (apoA-I), the major protein of HDL, was exposed to MPO or peroxynitrite …