Rapid identification and sorting of viable virus-reactive CD4+ and CD8+ T cells based on antigen-triggered CD137 expression
TC Wehler, M Karg, E Distler, A Konur, M Nonn… - Journal of …, 2008 - Elsevier
Current methods for the detection and isolation of antigen-specific CD4+ and CD8+ T cells
require the availability of peptide/MHC multimers or are restricted to cells that produce
cytokines after antigen contact. Here we show that de novo cell surface expression of the
TNF-receptor family member CD137 (4-1BB) identifies recently activated, but not resting,
human CD4+ and CD8+ memory T cells. Maximum CD137 expression level is uniformly
observed in both T-cell subsets at 24h after stimulation with antigen. In experiments with …
require the availability of peptide/MHC multimers or are restricted to cells that produce
cytokines after antigen contact. Here we show that de novo cell surface expression of the
TNF-receptor family member CD137 (4-1BB) identifies recently activated, but not resting,
human CD4+ and CD8+ memory T cells. Maximum CD137 expression level is uniformly
observed in both T-cell subsets at 24h after stimulation with antigen. In experiments with …
