Inhibition of lactate transport and glycolysis in Ehrlich ascites tumor cells by bioflavonoids
JA Belt, JA Thomas, RN Buchsbaum, E Racker - Biochemistry, 1979 - ACS Publications
JA Belt, JA Thomas, RN Buchsbaum, E Racker
Biochemistry, 1979•ACS PublicationsMaterials and Methods Ehrlich ascites tumor cells were maintained in male, white mice (Blu:
HA (ICR) Blue Spruce Farms, Inc., Altamont, NY) as described previously (Wu & Racker,
1959). Cells were harvested 7-10 days after injection by proceduresdescribed below.
Materials. A-2-Hydroxyethylpiperazine-./V/-2-ethanesulfonic acid (Hepes), 2-(/V-morpholino)
ethanesulfonic acid (Mes), 7V-tris (hydroxymethyl) methylglycine (Tricine),
morpholinopropanesulfonic acid (Mops), heparin, rotenone, bovine serum albumin, and L …
HA (ICR) Blue Spruce Farms, Inc., Altamont, NY) as described previously (Wu & Racker,
1959). Cells were harvested 7-10 days after injection by proceduresdescribed below.
Materials. A-2-Hydroxyethylpiperazine-./V/-2-ethanesulfonic acid (Hepes), 2-(/V-morpholino)
ethanesulfonic acid (Mes), 7V-tris (hydroxymethyl) methylglycine (Tricine),
morpholinopropanesulfonic acid (Mops), heparin, rotenone, bovine serum albumin, and L …
Materials and Methods
Ehrlich ascites tumor cells were maintained in male, white mice (Blu: HA (ICR) Blue Spruce Farms, Inc., Altamont, NY) as described previously (Wu & Racker, 1959). Cells were harvested 7-10 days after injection by proceduresdescribed below.
Materials. A-2-Hydroxyethylpiperazine-./V/-2-ethanesulfonic acid (Hepes), 2-(/V-morpholino) ethanesulfonic acid (Mes), 7V-tris (hydroxymethyl) methylglycine (Tricine), morpholinopropanesulfonic acid (Mops), heparin, rotenone, bovine serum albumin, and L-lactic acid were obtained from Sigma Chemical Co., St. Louis, MO. Tri-n-butyltin chloride was obtained fromAldrich Chemical Co., Milwaukee, WI; L-[U-14C] lactate,[14C] methylamine, methoxy [3H] inulin,[ca/-6o; c>’/-14C] inulin, 3H20, and [U-14C] sucrose were from New England Nuclear, Boston, MA; Versilube F50 and SF96 (50) were from General Electric Co., Silicone Products Dept., Waterford, NY, and aqueous counting scintillant (ACS) was from Amersham Corp., Arlington Heights, IL. All other chemicals were of reagent grade.
ACS Publications