[CITATION][C] Regulatory Mechanisms in Carbohydrate Metabolism: IV. PASTEUR EFFECT AND CRABTREE EFFECT IN ASCITES TUMOR CELLS
R Wu, E Racker - Journal of Biological Chemistry, 1959 - Elsevier
R Wu, E Racker
Journal of Biological Chemistry, 1959•ElsevierMaterials and Methods The general experimental procedures and methods were the same
as described in the preceding paper (41). Kinetic studies of glucose uptake and lactic acid
production were carried out in 50-ml. flasks with a fluid volume of 10 ml. at 30” in a Dubnoff
metabolic shaker. The flasks were fitted with rubber stoppers with glass tubing extending
into the flask as an inlet of air or Nz. Aliquots (0.5 or 1 ml.) were withdrawn at various time
intervals through an opening in the rubber stopper.
as described in the preceding paper (41). Kinetic studies of glucose uptake and lactic acid
production were carried out in 50-ml. flasks with a fluid volume of 10 ml. at 30” in a Dubnoff
metabolic shaker. The flasks were fitted with rubber stoppers with glass tubing extending
into the flask as an inlet of air or Nz. Aliquots (0.5 or 1 ml.) were withdrawn at various time
intervals through an opening in the rubber stopper.
Materials and Methods The general experimental procedures and methods were the same as described in the preceding paper (41). Kinetic studies of glucose uptake and lactic acid production were carried out in 50-ml. flasks with a fluid volume of 10 ml. at 30” in a Dubnoff metabolic shaker. The flasks were fitted with rubber stoppers with glass tubing extending into the flask as an inlet of air or Nz. Aliquots (0.5 or 1 ml.) were withdrawn at various time intervals through an opening in the rubber stopper.
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